Mouse monoclonal antibody, biotinylated

Anti-yENaC (y−subunit epithelial sodium channel)

CAT NO: CAM 005-02B | Application: IHC, ELISA

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Product Summary

Cat. No.

CAM 005-02B

Specificity

CAM 005-02 is specific for the inhibitory tract of human yENaC

Immunogen

The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

Clone Number

5c2

Epitope Specificity

The epitope is on the inhibitory tract of human yENaC. The epitope differs from that of CAM 003-07.

Unit Size

150 µL: Cat. No. CAM 005-02B-015 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Biotinylated

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide

Conjugation

Biotin

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the y-subunit and putative release of a 43-amino acid inhibitory tract from the y-subunit ectodomain.

Application

IHC: CAM 005-02B was used in IHC.

ELISA: CAM 005-02B was used in ELISA. A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).