Anti-Type V Collagen (human, dog, sheep, kangaroo, pig, rabbit, bovine)

CAT NO: CSI 006-01

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Product Name	Anti-Type V Collagen (human, dog, sheep, kangaroo, pig, rabbit, bovine)
Description	Mouse monoclonal antibody
Isotype	IgG2a/k
Host	Mouse
Clone	1E2-E4/Col5
Conjugation	Unconjugated
Tested Applications	ELISA, WB, IHC

ELISA	CSI 006-01 can be used for detection of collagens by ELISA. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
WB	In immunoblotting CSI 006-01 detects human type V collagen only in its native triple helix form.
IHC	CSI 006-01 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.

Specificity
Specificity	CSI 006-01 is highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with otherconnective tissue proteins (laminin, fibronectin, elastin).
Immunogen	Acid-digested pepsin soluble dog type V collagen
Target	Type V collagen is a minor component of the connective tissue, although it is present in many different types of connective tissue. Patients with defects in the type V collagen (Ehlers-Danlos syndrome) have weakend connective tissue characterized by hyperstrechable joints and fragile, easily bruisable skin.
Gene ID	1289,397533, 100848491, 480684
Species Reactivity POSITIVE	Human, Pig, Bovine, Dog, Sheep (ovine), Kangaroo, Rabbit
Species Reactivity NEGATIVE	Mouse, Rat, Guinea Pig, Chicken

Properties
Form	Liquid
Unit Size	0,4 mL and 1 mL
Concentration	1 mg/mL ±15%, See CoA for lot details
Purification	Protein A or Protein G purified
Purification Notes	BSA free
Storage buffer	0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

1. Pollock GA, McKelvie PA, McCarty DJ, White JF, Mallari PL, Taylor HR. (2003) In vivo effects of fluoroquinolones on rabbit corneas. Clin Experiment Ophthalmol 31:517-21.

2. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.

3. Werkmeister JA, Ramshaw JAM (1988) The use of immunohistology in studies on connective tissue organisation in hides and skins. Das Leder 39:145-151.

4. Werkmeister JA, Ramshaw JAM (1991) Monoclonal antibodies to type V collagen as markers for new tissue deposition associated with biomaterial implants. J Histochem Cytochem 39:1215-1220.

5. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.

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Anti-Type V Collagen (human, dog, sheep, kangaroo, pig, rabbit, bovine)

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