Mouse monoclonal antibody, biotinylated

Anti-Factor XII (human, heavy chain)

CAT NO: CAM 011-05B | Application: ELISA

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Product Summary

Cat. No.

CAM 011-05B

Specificity

CAM 011-05 binds the heavy chain of human factor XII, including factor XII derivatives such as α-factor XIIa and α-factor XIIa C1-inhibitor complexes.

Immunogen

Affinity purified human factor XII.

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

Clone Number

10-11-5

Epitope Specificity

Differs from CAM 011-37.

Unit Size

150 µL: Cat. No. CAM 111-05B-015 1 mL: Cat. No. CAM 111-05-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide

Preparation

Biotinylated

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Factor XII is a component in the coagulation cascade. It is a plasma glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII to a-factor XIIa, which in turn activates factor XI to factor XIa.

Application

ELISA: CAM 011-05B (approximately 2 μg/mL) was used as a detection antibody when used in combination with CAM 011-37 as a capture antibody (1-4 μg/mL) to measure Total factor XII, including factor XII derivatives such as α-factor XIIa and α-factor XIIa C1-inhibitor complexes.

Scientific References

1. Madsen DE, Sidelmann J, Overgaard K, Koch C, Brodersen Gram J (2013) ELISA for determination of total coagulation factor XII concentration in human plasma. J Imm Meth 394:32–9.