Mouse monoclonal antibody

Anti-NGAL (pig)

CAT NO: ABS 048-28 | Application: ELISA, WB, IHC-P

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Product Summary

Cat. No.

ABS 048-28

Specificity

ABS 048-28 binds pig NGAL (neutrophil gelatinase-associated lipocalin).

Immunogen

Recombinant pig NGAL

Host

Mouse

Positive Species Reactivity

Pig

Negative Species Reactivity

Human, Dog, Mouse, Rat

Isotype

IgG1/k

Gene ID

100153501

Clone Number

Epitope Specificity

Not determined

Unit Size

400 µL: Cat. No. ABS 048-28-04 1 mL: Cat. No. ABS 048-28-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Pig neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically, from renal tubular cells in response to ischemic or nephrotoxic injury.

Application

ELISA: ABS 048-28 binds free pig NGAL in solution. ABS 048-28 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 048-17 (as capture antibody) for measuring porcine NGAL.

WB: ABS 048-28 can be used in Western blotting.

IHC-P: ABS 048-28 detects NGAL in paraffin-embedded sections in the cytoplasm of renal tubular cell from pig.

NGAL expression in the ischemic injured pig kidney; detected with anti-pig NGAL antibody (ABS 048-28; antibody dilution 1:25), using the Ventana Immunostainer BENCHMARK XT from Roche. Image and data supplied by Dr. Despoina Karandrea, Aretaieion Hospotal, Athens, Greece.

Positive staining of renal tubular cell cytoplasm, using mouse monoclonal antibody against pig NGAL (Cat. no. ABS 048-28), dilution 1:1000. Courtesy of Oscar McCook, University of Ulm.