Mouse monoclonal antibody

Anti-NGAL (rat)

CAT NO: ABS 039-15 | Application: ELISA

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Product Summary

Cat. No.

ABS 039-15

Specificity

ABS 039-15 binds rat NGAL (neutrophil gelatinase-associated lipocalin).

Immunogen

Recombinant rat NGAL precursor with C-terminal His-tag coupled to carrier.

Host

Mouse

Positive Species Reactivity

Rat

Negative Species Reactivity

Human, Pig, Dog, Mouse

Isotype

IgG1/k

Gene ID

170496

Clone Number

Epitope Specificity

Not determined

Unit Size

400 µL: Cat. No. ABS 039-15-04 1 mL: Cat. No. ABS 039-15-1 1 mg/mL +/- 15%. See Certificate of Analysis for details9

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including tubular cells of the kidney. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is up-regulated in a variety of pathological situations including infection, inflammation, renal injury and certain adenocarcinomas.

Application

ELISA: ABS 039-15 binds free rat NGAL in solution. ABS 039-15 (as capture antibody) forms a sandwich ELISA pair with ABS 039-32 (as biotinylated detection antibody) for measuring rat NGAL (measuring range 4-400 pg/mL in an optimized assay).

Scientific References

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A