Anti-Perlecan (bovine, human)

CAT NO: CSI 001-74

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ELISA	CSI 001-74 can be used in ELISA.
WB	In Western blotting a dilution guideline of 1/100 has proved successful. (1)
IHC	CSI 001-74 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.
IP	CSI 001-74 can be used in immunoprecipitation.
AP	AP: CSI 001-74 can be used in affinity chromatography, in conjunction with CSI 001-71 to separate recombinant domain I from full-length perlecan. (1)

Specificity
Specificity	CSI 001-74 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).Epitope is located in domain V
Immunogen	Bovine corneal endothelial cells
Target	Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling.
Gene ID	444872, 3339
Species Reactivity POSITIVE	Bovine, Human
Species Reactivity NEGATIVE	Not determined

Properties
Form	Liquid
Unit Size	0,4 mL and 1 mL
Concentration	1 mg/mL ±15%, See CoA for lot details
Purification	Protein A or Protein G purified
Purification Notes	BSA free
Storage buffer	0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide