Mouse monoclonal antibody

Anti-Vitronectin (human, dog, cat, goat, horse, bovine)

CAT NO: CSI 003-23 | Application: ELISA, WB

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Product Summary

Cat. No.

CSI 003-23

Specificity

CSI 003-23 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 003-23 binds nearly as well to native vitronectin as to denatured. CSI 003-23 is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding.

Immunogen

Human vitronectin purified from plasma by heparin-affinity chromatography

Host

Mouse

Positive Species Reactivity

Human, Cat (feline), Dog, Goat (caprine), Bovine, Horse

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

7448, 101100765, 480621, 100861216, 507525, 100059034

Clone Number

HV23

Epitope Specificity

Epitope is located in the somatomedin B domain

Unit Size

400 µL: Cat. No. CSI 003-23-04 1 mL: Cat. No. CSI 003-23-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognizes fibronectin. (2)

Application

ELISA: CSI 003-23 also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well. In Western blotting a dilution guideline of 1/50 and 1/200 has proved successful. (1,2)

WB: CSI 003-23 can be used in Western blotting. (1)

Scientific References

1. Morris CA, Underwood PA, Bean PA, Sheehan M, Charlesworth JA (1994) Relative topography of biologically active domains of human vitronectin. Evidence from monoclonal antibody epitope and denaturation studies. J Biol Chem 269:23845-23852.

2. Underwood PA, Kirkpatrick A, Mitchell SM (2002) New insights into heparin binding to vitronectin: studies with monoclonal antibodies. Biochem J 365:57-67.