Mouse monoclonal antibody

Anti-Vitronectin (bovine, sheep)

CAT NO: CSI 004-27 | Application: ELISA, WB, IHC-F, IHC-P, AP

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Product Summary

Cat. No.
CSI 004-27
Specificity
CSI 004-27 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).
Immunogen
Lysed bovine corneal endothelial cells and extracellular matrix
Host
Mouse
Positive Species Reactivity
Bovine, Sheep (ovine)
Negative Species Reactivity
Human, Horse
Isotype
IgG1/k
Gene ID
507,525,101,119,231
Clone Number
A27
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. CSI 004-27-04 1 mL: Cat. No. CSI 004-27-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin. (1)

Application

ELISA: CSI 004-27 is suitable for ELISA. (1, 2, 3)

WB: CSI 004-27 is suitable for immunoblotting.

IHC-F: CSI 004-27 immunostaining of frozen PLP-fixed sections of bovine tissues.

IHC-P: CSI 004-27 immunostaining of frozen PLP-fixed sections of bovine tissues.

AP: The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation.

Scientific References

1. Underwood PA, Bennett FA (1989) A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin. J Cell Sci 93:641-649.

2. Underwood PA, Steele JG, Dalton BA, Bennet FA (1990). Solid phase monoclonal antibodies. A novel method of directing the function of biologically active molecules be presenting a specific concentration. J Immunol Methods 127:91-102.

3. Underwood PA, Bean PA, Mitchell SM, Whitelock JM (2001) Specific affinity depletion of cell adhesion molecules and growth factors from serum. J Immunol Methods 247:217-224.

Cat. No.
CSI 004-27
Specificity
CSI 004-27 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).
Immunogen
Lysed bovine corneal endothelial cells and extracellular matrix
Host
Mouse
Positive Species Reactivity
Bovine, Sheep (ovine)
Negative Species Reactivity
Human, Horse
Isotype
IgG1/k
Gene ID
507,525,101,119,231
Clone Number
A27
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. CSI 004-27-04 1 mL: Cat. No. CSI 004-27-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin. (1)

ELISA: CSI 004-27 is suitable for ELISA. (1, 2, 3)

WB: CSI 004-27 is suitable for immunoblotting.

IHC-F: CSI 004-27 immunostaining of frozen PLP-fixed sections of bovine tissues.

IHC-P: CSI 004-27 immunostaining of frozen PLP-fixed sections of bovine tissues.

AP: The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation.

1. Underwood PA, Bennett FA (1989) A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin. J Cell Sci 93:641-649.

2. Underwood PA, Steele JG, Dalton BA, Bennet FA (1990). Solid phase monoclonal antibodies. A novel method of directing the function of biologically active molecules be presenting a specific concentration. J Immunol Methods 127:91-102.

3. Underwood PA, Bean PA, Mitchell SM, Whitelock JM (2001) Specific affinity depletion of cell adhesion molecules and growth factors from serum. J Immunol Methods 247:217-224.

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Product Specification

Non-hazardous statement (SDS)

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