Mouse monoclonal antibody

Anti-Vitronectin (bovine, sheep)

CAT NO: CSI 004-27 | Application: ELISA, WB, IHC-F, IHC-P, AP

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Product Summary

Cat. No.

CSI 004-27

Specificity

CSI 004-27 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).

Immunogen

Lysed bovine corneal endothelial cells and extracellular matrix

Host

Mouse

Positive Species Reactivity

Bovine, Sheep (ovine)

Negative Species Reactivity

Human, Horse

Isotype

IgG1/k

Gene ID

507,525,101,119,231

Clone Number

A27

Epitope Specificity

Not determined

Unit Size

400 µL: Cat. No. CSI 004-27-04 1 mL: Cat. No. CSI 004-27-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin. (1)

Application

ELISA: CSI 004-27 is suitable for ELISA. (1, 2, 3)

WB: CSI 004-27 is suitable for immunoblotting.

IHC-F: CSI 004-27 immunostaining of frozen PLP-fixed sections of bovine tissues.

IHC-P: CSI 004-27 immunostaining of frozen PLP-fixed sections of bovine tissues.

AP: The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation.

Scientific References

1. Underwood PA, Bennett FA (1989) A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin. J Cell Sci 93:641-649.

2. Underwood PA, Steele JG, Dalton BA, Bennet FA (1990). Solid phase monoclonal antibodies. A novel method of directing the function of biologically active molecules be presenting a specific concentration. J Immunol Methods 127:91-102.

3. Underwood PA, Bean PA, Mitchell SM, Whitelock JM (2001) Specific affinity depletion of cell adhesion molecules and growth factors from serum. J Immunol Methods 247:217-224.