Mouse monoclonal antibody

Anti-Vitronectin (human)

CAT NO: CSI 003-02 | Application: ELISA, WB, IHC, AP

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Product Summary

Cat. No.
CSI 003-02
Specificity
Vitronectin (human)
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Cat, Dog, Cow, Sheep, Goat, Pig, Rabbit, Horse
Isotype
IgG2b/k
Gene ID
7448
Clone Number
HV2
Epitope Specificity
The epitope is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133.
Unit Size
400 µL: Cat. No. CSI 003-02-04 1 mL: Cat. No. CSI 003-02-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognizes fibronectin. (2)

Application

ELISA: It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-08. It does not interfere with the binding of any known vitronectin ligands. CSI 003-02 also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well. In Western blotting a dilution guideline of 1/100 has proved successful. (1,2)

WB: CSI 003-02 can be used in Western blotting. (1, 2)

Independent review from antibodyresource.com.

IHC: CSI 003-02 can be used in IHC. Please consult www.proteinatlas.org

Paraffin-embedded human cancerous liver tissue immunostained with CSI 003-02. (www.proteinatlas.org)

AP: CSI 003-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding.

Scientific References

1. Morris CA, Underwood PA, Bean PA, Sheehan M, Charlesworth JA (1994) Relative topography of biologically active domains of human vitronectin. Evidence from monoclonal antibody epitope and denaturation studies. J Biol Chem 269:23845-23852.

2. Underwood PA, Kirkpatrick A, Mitchell SM (2002) New insights into heparin binding to vitronectin: studies with monoclonal antibodies. Biochem J 365:57-67.

Cat. No.
CSI 003-02
Specificity
Vitronectin (human)
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Cat, Dog, Cow, Sheep, Goat, Pig, Rabbit, Horse
Isotype
IgG2b/k
Gene ID
7448
Clone Number
HV2
Epitope Specificity
The epitope is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133.
Unit Size
400 µL: Cat. No. CSI 003-02-04 1 mL: Cat. No. CSI 003-02-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognizes fibronectin. (2)

ELISA: It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-08. It does not interfere with the binding of any known vitronectin ligands. CSI 003-02 also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well. In Western blotting a dilution guideline of 1/100 has proved successful. (1,2)

WB: CSI 003-02 can be used in Western blotting. (1, 2)

Independent review from antibodyresource.com.

IHC: CSI 003-02 can be used in IHC. Please consult www.proteinatlas.org

Paraffin-embedded human cancerous liver tissue immunostained with CSI 003-02. (www.proteinatlas.org)

AP: CSI 003-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding.

1. Morris CA, Underwood PA, Bean PA, Sheehan M, Charlesworth JA (1994) Relative topography of biologically active domains of human vitronectin. Evidence from monoclonal antibody epitope and denaturation studies. J Biol Chem 269:23845-23852.

2. Underwood PA, Kirkpatrick A, Mitchell SM (2002) New insights into heparin binding to vitronectin: studies with monoclonal antibodies. Biochem J 365:57-67.

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Product Specification

Non-hazardous statement (SDS)

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