Mouse monoclonal antibody

Anti-Vitronectin (human)

CAT NO: CSI 003-02 | Application: ELISA, WB, IHC, AP

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Product Summary

Cat. No.

CSI 003-02

Specificity

Vitronectin (human)

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Cat, Dog, Cow, Sheep, Goat, Pig, Rabbit, Horse

Isotype

IgG2b/k

Gene ID

7448

Clone Number

HV2

Epitope Specificity

The epitope is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133.

Unit Size

400 µL: Cat. No. CSI 003-02-04 1 mL: Cat. No. CSI 003-02-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognizes fibronectin. (2)

Application

ELISA: It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-08. It does not interfere with the binding of any known vitronectin ligands. CSI 003-02 also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well. In Western blotting a dilution guideline of 1/100 has proved successful. (1,2)

WB: CSI 003-02 can be used in Western blotting. (1, 2)

Independent review from antibodyresource.com.

IHC: CSI 003-02 can be used in IHC. Please consult www.proteinatlas.org

Paraffin-embedded human cancerous liver tissue immunostained with CSI 003-02. (www.proteinatlas.org)

AP: CSI 003-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding.

Scientific References

1. Morris CA, Underwood PA, Bean PA, Sheehan M, Charlesworth JA (1994) Relative topography of biologically active domains of human vitronectin. Evidence from monoclonal antibody epitope and denaturation studies. J Biol Chem 269:23845-23852.

2. Underwood PA, Kirkpatrick A, Mitchell SM (2002) New insights into heparin binding to vitronectin: studies with monoclonal antibodies. Biochem J 365:57-67.