Mouse monoclonal antibody
Anti-Vitronectin (human)
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Product Summary
Application
ELISA: It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-08. It does not interfere with the binding of any known vitronectin ligands. CSI 003-02 also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well. In Western blotting a dilution guideline of 1/100 has proved successful. (1,2)
WB: CSI 003-02 can be used in Western blotting. (1, 2)
IHC: CSI 003-02 can be used in IHC. Please consult www.proteinatlas.org
AP: CSI 003-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding.
Scientific References
1. Morris CA, Underwood PA, Bean PA, Sheehan M, Charlesworth JA (1994) Relative topography of biologically active domains of human vitronectin. Evidence from monoclonal antibody epitope and denaturation studies. J Biol Chem 269:23845-23852.
2. Underwood PA, Kirkpatrick A, Mitchell SM (2002) New insights into heparin binding to vitronectin: studies with monoclonal antibodies. Biochem J 365:57-67.
ELISA: It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-08. It does not interfere with the binding of any known vitronectin ligands. CSI 003-02 also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well. In Western blotting a dilution guideline of 1/100 has proved successful. (1,2)
WB: CSI 003-02 can be used in Western blotting. (1, 2)
IHC: CSI 003-02 can be used in IHC. Please consult www.proteinatlas.org
AP: CSI 003-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding.
1. Morris CA, Underwood PA, Bean PA, Sheehan M, Charlesworth JA (1994) Relative topography of biologically active domains of human vitronectin. Evidence from monoclonal antibody epitope and denaturation studies. J Biol Chem 269:23845-23852.
2. Underwood PA, Kirkpatrick A, Mitchell SM (2002) New insights into heparin binding to vitronectin: studies with monoclonal antibodies. Biochem J 365:57-67.
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