Mouse monoclonal antibody, biotinylated

Anti-GLP-1(7-37) and GLP-1(7-36) amide (N-terminal specific)

CAT NO: ABS 033-10B | Application: ELISA

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Product Summary

Cat. No.
ABS 033-10B
Specificity
Glucagon-like peptide-1 (GLP-1(7-37) and GLP-1(7-36)amide, N-terminal specific)
Immunogen
Synthetic GLP-1(7-17)
Host
Mouse
Positive Species Reactivity
Mammalia
Negative Species Reactivity
Not determined
Isotype
IgG2a/k
Gene ID
2641
Clone Number
10
Epitope Specificity
Free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide.
Unit Size
150 µL: Cat. No. ABS 033-10B-015 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide
Conjugation
Biotin
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Application

ELISA: ABS 033-10B can be used as a detection antibody in combination with ABS 046-03 as a capture antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.

Cat. No.
ABS 033-10B
Specificity
Glucagon-like peptide-1 (GLP-1(7-37) and GLP-1(7-36)amide, N-terminal specific)
Immunogen
Synthetic GLP-1(7-17)
Host
Mouse
Positive Species Reactivity
Mammalia
Negative Species Reactivity
Not determined
Isotype
IgG2a/k
Gene ID
2641
Clone Number
10
Epitope Specificity
Free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide.
Unit Size
150 µL: Cat. No. ABS 033-10B-015 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide
Conjugation
Biotin
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

ELISA: ABS 033-10B can be used as a detection antibody in combination with ABS 046-03 as a capture antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.

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Product Specification

Non-hazardous statement (SDS)

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