Mouse monoclonal antibody

Anti-Gastric Inhibitory Peptide (3-42) (GIP (3-42))

CAT NO: ABS 057-25 | Application: ELISA

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Product Summary

Cat. No.

ABS 057-25

Specificity

ABS 057-25 binds human GIP (3-42).

Immunogen

Synthetic human gastric inhibitory polypeptide

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Gene ID

2695

Clone Number

Epitope Specificity

The epitope lies within the N-terminal region of GIP (3-42)

Unit Size

400 µL: Cat. No. ABS 057-25-04 1 mL: Cat. No. ABS 057-25-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

2-8ºC without exposure to light. No precautions necessary during handling.

Target

Human gastric inhibitory polypeptide (GIP) is a 42 amino acid peptide belonging to the glucagon-secretin family of peptide hormones. It is secreted by endocrine cells in the duodenal mucosa and stimulates glucose-dependent insulin secretion as well as GLP-1 release from more distal endocrine (L) cells in the intestinal mucosa. GIP shows amino-acid sequence similarities to glucagon, GLP-1 and GLP-2 (from approximately 50% identity for glucagon to 30% identity for GLP-2).

Application

ELISA: ABS 057-25 (as capture antibody) forms a sandwich ELISA pair with ABS 054-14B (as biotinylated detection antibody) for measuring GIP. Data suggest that this matched antibody pair is the most sensitive pair for measuring GIP (3-42). The detection limit obtained with our non-optimized buffer system is up to 1 ng/mL of GIP (3-42). Cross-reaction with GIP (1-42) is around 2%.