Mouse monoclonal antibody

Anti-Glucagon (human, pig, rat)

CAT NO: ABS 061-09 | Application: ELISA, IHC, IF

Request Price

"*" indicates required fields

Name*

First Last

Email*

Organization

Shipping Address*

City, State, Post Code/Zip*

Country*

Phone

Catalog Number ( Product )

Message

This field is for validation purposes and should be left unchanged.

Product Summary

Cat. No.

ABS 061-09

Specificity

ABS 061-09 binds human glucagon. Cross-reacts less than 5% with oxyntomodulin.

Immunogen

Synthetic full length oxyntomodulin coupled to carrier.

Host

Mouse

Positive Species Reactivity

Human, Rat, Pig

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

2641, 24952, 397595

Clone Number

Epitope Specificity

The epitope lies within the C-terminal region of glucagon.

Unit Size

400 µL: Cat. No. ABS 061-09-04 1 mL: Cat. No. ABS 061-09-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Glucagon is a peptide hormone of 29 amino-acid residues produced by the alpha cells of the pancreatic islets. Its effect is to raise blood sugar as a stress hormone and counter-regulatory hormone for insulin. It is produced by cell-type-specific processing of proglucagon, which in the alpha cells is split to form glicentin-related pancreatic polypeptide, glucagon and major proglucagon fragment, while the same precursor is split to form glicentin, oxyntomodulin (C-terminally extended glucagon-37), GLP-1 and GLP-2 in the L cells of the intestinal mucosa.

Application

ELISA: ABS 061-09 binds free glucagon in solution. ABS 061-09 when used as a capture antibody forms a sandwich ELISA pair with ABS 058-08 as a biotinylated detection antibody for measuring glucagon.

IHC: ABS 061-09 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:8000.

Formalin fixed paraffin-embedded human pancreas tissue stained with ABS 061-09. Alpha cells of the Langerhans islet were stained strongly. Dilution: 1:8000

IF: ABS 061-09 was used in IF with a dilution of 1:4000.

IF staining in human pancreas tissue stained with ABS 061-09. Signal is strong in alpha cells. Dilution: 1:4000.