Anti-Pregnancy associated plasma protein A (human, PAPP-A)

CAT NO: BTE 004-09

Request Price

"*" indicates required fields

Instagram

This field is for validation purposes and should be left unchanged.

Name*

First Last

Email*

Phone

Organization

Shipping Address*

City, State, Post Code/Zip*

Country*

States*

Catalog Number ( Product )

Message

Product Name	Anti-Pregnancy associated plasma protein A (human, PAPP-A)
Description	Mouse monoclonal antibody
Isotype	IgG2b/k
Host	Mouse
Clone	9
Conjugation	Unconjugated
Tested Applications	ELISA, WB, IHC

ELISA	In sandwich ELISA the optimal combination is BTE 004-09 used as coating antibody and biotinylated ABS 006-24 used as a detection antibody. BTE 004-09 can also be used as a detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat.
WB	BTE 004-09 detect both reduced and unreduced PAPP-A in Western Blotting, though the reaction to unreduced is less than that of ABS 006-01 and ABS 006-24.
IHC	BTE 004-09 can be used in IHC.
	Staining is restricted to the syncytiotrophoblast of the chorionic villi in frozen human placenta tissue, stained with BTE 004-09.	Staining is restricted to the syncytiotrophoblast of the chorionic villi in paraffin embedded human placenta tissue, stained with BTE 004-09.

Specificity
Specificity	BTE 004-09 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.The epitope of BTE 004-09 is different from ABS 006-01 and ABS 006-24. The epitope is located on the PAPP-A subunit of the heterotetramer.
Immunogen	Purified human PAPP-A
Target	Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).
Gene ID	5069
Species Reactivity POSITIVE	Human
Species Reactivity NEGATIVE	Not determined

Properties
Form	Liquid
Unit Size	0,4 mL and 1 mL
Concentration	1 mg/mL ±15%, See CoA for lot details
Purification	Protein A or Protein G purified
Purification Notes	BSA free
Storage buffer	0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide