Mouse monoclonal antibody

Anti-Pregnancy associated plasma protein A (human, PAPP-A)

CAT NO: BTE 004-09 | Application: ELISA, WB, IHC-P, IHC-F

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Product Summary

Cat. No.

BTE 004-09

Specificity

BTE 004-09 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.

Immunogen

Purified human PAPP-A

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

IgG2b/k

Gene ID

5069

Clone Number

Epitope Specificity

The epitope of BTE 004-09 is different from ABS 006-01 and ABS 006-24. The epitope is located on the PAPP-A subunit of the heterotetramer.

Unit Size

400 µL: Cat. No. BTE 004-09-04 1 mL: Cat. No. BTE 004-09-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).

Application

ELISA: In sandwich ELISA the optimal combination is BTE 004-09 used as coating antibody and biotinylated ABS 006-24 used as a detection antibody. BTE 004-09 can also be used as a detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat.

WB: BTE 004-09 detect both reduced and unreduced PAPP-A in Western Blotting, though the reaction to unreduced is less than that of ABS 006-01 and ABS 006-24.

IHC-P: BTE 004-09 can be used in IHC.

IHC-F: BTE 004-09 can be used in IHC.

Staining is restricted to the syncytiotrophoblast of the chorionic villi in frozen human placenta tissue, stained with BTE 004-09.

Staining is restricted to the syncytiotrophoblast of the chorionic villi in paraffin embedded human placenta tissue, stained with BTE 004-09.