Anti-Vitronectin (bovine, sheep)

CAT NO: CSI 004-27

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Product Name	Anti-Vitronectin (bovine, sheep)
Description	Mouse monoclonal antibody
Isotype	IgG1/k
Host	Mouse
Clone	A27
Conjugation	Unconjugated
Tested Applications	ELISA, WB, IHC, AP

ELISA	CSI 004-27 is suitable for ELISA. (1, 2, 3)
WB	CSI 004-27 is suitable for immunoblotting.
IHC	CSI 004-27 immunostaining of frozen PLP-fixed sections of bovine tissues.
AP	The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation.

Specificity
Specificity	CSI 004-27 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).
Immunogen	Lysed bovine corneal endothelial cells and extracellular matrix
Target	Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin. (1)
Gene ID	507525, 101119231
Species Reactivity POSITIVE	Bovine, Sheep (ovine)
Species Reactivity NEGATIVE	Human, Horse

Properties
Form	Liquid
Unit Size	0,4 mL and 1 mL
Concentration	1 mg/mL ±15%, See CoA for lot details
Purification	Protein A or Protein G purified
Purification Notes	BSA free
Storage buffer	0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide