Product Summary

Cat. No.
ABS 052-121B
Specificity
ABS 052-121 binds human ghrelin.
Immunogen
Synthetic ghrelin (14-28)
Host
Mouse
Positive Species Reactivity
Human, Rat, Mouse, Dog, Cat
Negative Species Reactivity
Not determined
Isotype
IgG2b/k
Gene ID
51738, 59301
Clone Number
121
Epitope Specificity
Not determined
Unit Size
150 µL: Cat. No. ABS 052-121B-015 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide
Conjugation
Biotin
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food reflect those of active ghrelin.

Application

ELISA: ABS 052-121 (as biotinylated detection antibody) forms an antibody pair with ABS 050-45 (as capture antibody) for measuring human ghrelin in sandwich ELISA.

Cat. No.
ABS 052-121B
Specificity
ABS 052-121 binds human ghrelin.
Immunogen
Synthetic ghrelin (14-28)
Host
Mouse
Positive Species Reactivity
Human, Rat, Mouse, Dog, Cat
Negative Species Reactivity
Not determined
Isotype
IgG2b/k
Gene ID
51738, 59301
Clone Number
121
Epitope Specificity
Not determined
Unit Size
150 µL: Cat. No. ABS 052-121B-015 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide
Conjugation
Biotin
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food reflect those of active ghrelin.

ELISA: ABS 052-121 (as biotinylated detection antibody) forms an antibody pair with ABS 050-45 (as capture antibody) for measuring human ghrelin in sandwich ELISA.

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