Mouse monoclonal antibody

Anti-Ghrelin (human)

CAT NO: ABS 050-45 | Application: ELISA, WB

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Product Summary

Cat. No.

ABS 050-45

Specificity

ABS 050-45 binds human octanoylated ghrelin and human des-acyl ghrelin.

Immunogen

Synthetic human Ser3-octanoyl ghrelin-28

Host

Mouse

Positive Species Reactivity

Human, Rat (5%)

Negative Species Reactivity

Not determined

Isotype

IgG1/l

Gene ID

51738

Clone Number

Epitope Specificity

Not determined. Probably includes residues 11 or 12 to account for the lower cross-reactivity with rat ghrelin.

Unit Size

400 µL: Cat. No. ABS 050-45-04 1 mL: Cat. No. ABS 050-45-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food may reflect those of active ghrelin.

Application

ELISA: ABS 050-45 binds human ghrelin in solution. ABS 050-45 (as a capture antibody) forms a pair with ABS 052-121 as a biotinylated detection antibody for measuring total human ghrelin by sandwich ELISA.

WB: ABS 050-45 can be used in Western blotting.