Mouse monoclonal antibody

Anti-PYY(3-36) (peptide tyrosine-tyrosine amide, 3-36)

CAT NO: ABS 051-04 | Application: ELISA, WB

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Product Summary

Cat. No.

ABS 051-04

Specificity

ABS 051-04 binds human PYY(3-36). Based on inhibition ELISA, ABS 051-04 shows no cross-reaction with PYY(1-36) or NPY.

Immunogen

Synthetic N-terminal fragment of human PYY(3-36) coupled to carrier.

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Mouse, Rat, Dog, Pig

Isotype

IgG2a/k

Gene ID

5697

Clone Number

Epitope Specificity

The epitope lies within residues 3-12 of human PYY.

Unit Size

400 µL: Cat. No. ABS 051-04-04 1 mL: Cat. No. ABS 051-04-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Peptide tyrosine-tyrosine amide (3-36), or PYY(3-36), is a major metabolite of the gut hormone PYY and is produced by the action of dipeptidyl peptidase IV on PYY in the intestinal brush border and in the circulation. PYY(3-36) is a potent inhibitor of food intake in rats and humans, acting selectively on the Y2 receptor to inhibit orexigenic NPY neurons in the arcuate nucleus, thus disinhibiting anorexigenic POMC neurons. The anorexigenic effect of PYY(3-36) is additive to that of the co-secreted gut hormone GLP-1.

Application

ELISA: ABS 051-04 binds free PYY(3-36) in solution. An ELISA dilution guideline of 1/20,000 applies to PYY(3-36) coated directly at 1 μg/mL. ABS 051-04 as a capture antibody forms a sandwich ELISA antibody pair with biotinylated ABS 029-01 as a detection antibody for measuring PYY(3-36).

WB: ABS 051-04 can be used in Western blotting.