Anti-Ghrelin (human)

CAT NO: ABS 050-45

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ELISA	ABS 050-45 binds human ghrelin in solution. ABS 050-45 (as a capture antibody) forms a pair with ABS 052-121 as a biotinylated detection antibody for measuring total human ghrelin by sandwich ELISA.
WB	ABS 050-45 can be used in Western blotting.

Specificity
Specificity	ABS 050-45 binds human octanoylated ghrelin and human des-acyl ghrelin.
Immunogen	Synthetic human Ser3-octanoyl ghrelin-28
Target	Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food may reflect those of active ghrelin.
Gene ID	51738
Species Reactivity POSITIVE	Human, Rat (5%)
Species Reactivity NEGATIVE	Not determined

Properties
Form	Liquid
Unit Size	0,4 mL and 1 mL
Concentration	1 mg/mL ±15%, See CoA for lot details
Purification	Protein A or Protein G purified
Purification Notes	BSA free
Storage buffer	0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide